AG07680
Endothelial from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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60,XY
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
90 |
| PDL at Freeze |
27 |
| Passage Frozen |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
Holstein breed. The thoracic aorta was obtained on 4/25/79 from a 4 to 5 month old fetus. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. Plasma-derived serum was used in the primary culture to select against possible smooth muscle cell contaminants. The cell morphology is polygonal. The karyotype is 60,XY; normal diploid male. This culture tested positive for the endothelial cellspecific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays) and positive for angiotensin-1 converting enzyme activity. Culture tested negative for bovine viruses BVD, PI-3 and BHV-1 prior to submission to the Repository. Same culture, line BFAE-39, at later passage is AG04762A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cullen JP, Sayeed S, Sawai RS, Theodorakis NG, Cahill PA, Sitzmann JV, Redmond
EM, Pulsatile flow-induced angiogenesis: role of G(i) subunits. Arterioscler Thromb Vasc Biol22(10):1610-6 2002 |
| PubMed ID: 12377738 |
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| Liu XD, Skold M, Umino T, Zhu YK, Romberger DJ, Spurzem JR, Rennard SI, Endothelial cell-mediated type I collagen gel contraction is regulated by hemin. J Lab Clin Med136(2):100-9 2000 |
| PubMed ID: 10945238 |
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| Alexander JS, Patton WF, Christman BW, Cuiper LL, Haselton FR, Platelet-derived lysophosphatidic acid decreases endothelial permeability in vitro. Am J Physiol274:H115-22 1998 |
| PubMed ID: 9458859 |
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| Redmond EM, Cahill PA, Sitzmann JV, Flow-mediated regulation of G-protein expression in cocultured vascular smooth muscle and endothelial cells. Arterioscler Thromb Vasc Biol18:75-83 1998 |
| PubMed ID: 9445259 |
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| Ziegelstein RC, Corda S, Pili R, Passaniti A, Lefer D, Zweier JL, Fraticelli A, Capogrossi MC, Initial contact and subsequent adhesion of human neutrophils or monocytes to human aortic endothelial cells releases an endothelial intracellular calcium store. Circulation90(4):1899-907 1994 |
| PubMed ID: 7923678 |
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| Joshi P, Chung CY, Aukhil I, Erickson HP, Endothelial cells adhere to the RGD domain and the fibrinogen-like terminal knob of tenascin. J Cell Sci106 ( Pt 1):389-400 1993 |
| PubMed ID: 7505785 |
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| Pili R, Corda S, Passaniti A, Ziegelstein RC, Heldman AW, Capogrossi MC, Endothelial cell Ca2+ increases upon tumor cell contact and modulates cell-cell adhesion. J Clin Invest92(6):3017-22 1993 |
| PubMed ID: 8254056 |
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| Presta M, Maier JA, Rusnati M, Ragnotti G, Basic fibroblast growth factor: production, mitogenic response, and post-receptor signal transduction in cultured normal and transformed fetal bovine aortic endothelial cells. J Cell Physiol141:517-26 1989 |
| PubMed ID: 2556410 |
| PDL at Senescence |
90 |
| Cumulative PDL at Freeze |
27 |
| Passage Frozen |
8 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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