AG07980
Smooth muscle from Aorta, thoracic
Description:
EQUUS
ORDER: PERISSODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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64,XY
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Species
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Equus caballus
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Common Name
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horse
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Remarks
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| PDL at Senescence |
64 |
| PDL at Freeze |
14 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Standardbred horse. The thoracic aorta was removed immediately post-mortem on 12/7/84. Strips of the medial layer were minced, treated with collagenase and then elastase. Cells released by this treatment were used for the primary culture. Cell morphology is fibroblast-like and the cells form layered sheets at post-confluence. The karyotype is 64,XY; normal diploid male. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). For lifespan determination this cell line was cultured in Ham's F12 medium and MCDB104 medium in a 1/1 mixture with 10% inactivated fetal bovine serum and supplements (0.04-0.06mg/ml crude ECGF and 0.05mg/ml heparin). A skin fibroblast culture from this horse is AG07984. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| PDL at Senescence |
64 |
| Cumulative PDL at Freeze |
14 |
| Passage Frozen |
3 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) with 2X conc. A.A. and VIT./MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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