AG08076
Fibroblast from Lung, Lung
Description:
CANIS
ORDER: CARNIVORA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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78,XX
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Species
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Canis familiaris
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Common Name
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dog
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Remarks
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| PDL at Senescence |
15 |
| PDL at Freeze |
2 |
| Passage Frozen |
1 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Beagle breed. The lung biopsy was taken post-mortem on 3/19/85. The culture was initiated using explants of minced lung tissue. Cell morphology is fibroblast-like. The karyotype is 78,XX; normal diploid female. A matching skin fibroblast culture from this dog is AG08075. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| PDL at Senescence |
15 |
| Cumulative PDL at Freeze |
2 |
| Passage Frozen |
1 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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