AG08080
Fibroblast from Skin, Thigh
Description:
EQUUS
ORDER: PERISSODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thigh
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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64,XY
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Species
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Equus caballus
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Common Name
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horse
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Remarks
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| PDL at Freeze |
3 |
| Passage Frozen |
1 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
Quarter horse breed. The skin biopsy was taken post-mortem on 3/21/85 from the inner thigh. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. The karyotype is 64,XY; normal diploid male. A smooth muscle cell culture from this horse is AG08082. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Murphy WJ, Fronicke L, O'Brien SJ, Stanyon R, The origin of human chromosome 1 and its homologs in placental mammals. Genome Res13(8):1880-8 2003 |
| PubMed ID: 12869576 |
| Cumulative PDL at Freeze |
3 |
| Passage Frozen |
1 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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