AG08130
Fibroblast from Skin, Thigh
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thigh
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Freeze |
10 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
Holstein breed. The skin biopsy was taken 4 hours post-mortem on 4/18/85 from the inner thigh. Culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. The karyotype is 60,XX; normal diploid female. Other cultures from this cow cow are AG08131A, AG08132B, and AG08133A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cohick WS, Wang B, Verma P, Boisclair YR, Insulin-Like growth factor I (IGF-I) and cyclic adenosine 3',5'-monophosphate
regulate IGF-binding protein-3 gene expression by transcriptional and
posttranscriptional mechanisms in mammary epithelial cells. Endocrinology141(12):4583-91 2000 |
| PubMed ID: 11108271 |
| Cumulative PDL at Freeze |
10 |
| Passage Frozen |
5 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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