AG08133
Smooth muscle from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
83 |
| PDL at Freeze |
7 |
| Passage Frozen |
6 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Holstein breed. The thoracic aorta was removed 4 hours post-mortem on 4/18/85. Strips of the medial layer were removed, minced, and used as explants to initiate the culture. The cell morphology is fibroblast-like. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). Other cultures from this cow are AG08130, AG08131A, and AG08132B. |
| Auge N, Andrieu N, Negre-Salvayre A, Thiers JC, Levade T, Salvayre R, The sphingomyelin-ceramide signaling pathway is involved in oxidized low density lipoprotein-induced cell proliferation. J Biol Chem271:19251-5 1996 |
| PubMed ID: 8702606 |
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| Auge N, Fitoussi G, Bascands JL, Pieraggi MT, Junquero D, Valet P, Girolami JP, Salvayre R, Negre-Salvayre A, Mildly oxidized LDL evokes a sustained Ca(2+)-dependent retraction of vascular smooth muscle cells. Circ Res79:871-80 1996 |
| PubMed ID: 8831513 |
| PDL at Senescence |
83 |
| Cumulative PDL at Freeze |
7 |
| Passage Frozen |
6 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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