AG08137
Fibroblast from Skin, Arm
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Italian Alzheimer Disease |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Ethnicity
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ITALIAN
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Family Member
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75
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Relation to Proband
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VI-1214
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
7 |
| Passage Frozen |
8 |
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| Remarks |
The donor is 50% at risk for Alzheimer's disease. The skin biopsy was taken ante-mortem from the forearm. Culture was initiated on 4/26/85 using explants of minced skin. The cell morphology is fibroblast-like. Culture was frozen at PDL 7. A lymphoblast culture from same donor is AG08136. |
| St George-Hyslop PH, Tanzi RE, Polinsky RJ, Haines JL, Nee L, Watkins PC, Myers RH, Feldman RG, Pollen D, Drachman D, et al, The genetic defect causing familial Alzheimer's disease maps on chromosome 21. Science235:885-90 1987 |
| PubMed ID: 2880399 |
| |
| Foncin, Presenile Alzheimer's disease in a large kindred. Rev Neurol141:194 (1985):885-90 1985 |
| PubMed ID: 4001707 |
| Cumulative PDL at Freeze |
7 |
| Passage Frozen |
8 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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