AG08182
LCL from B-Lymphocyte
Description:
ALZHEIMER DISEASE, FAMILIAL, TYPE 3
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Canadian Alzheimer Disease |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Ethnicity
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CANADIAN
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Family Member
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6
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Relation to Proband
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VI-44
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Confirmation
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Clinical summary/Case history
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ISCN
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45,X/46,XX
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The donor is a probable escapee from Alzheimer's disease. The culture was initiated on 05/17/85 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. The culture is a mosaic with karyotype: 45,X/46,XX; unbalanced; 12%/88%. A skin fibroblast culture from same donor is AG08183. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| St George-Hyslop PH, Tanzi RE, Polinsky RJ, Haines JL, Nee L, Watkins PC, Myers RH, Feldman RG, Pollen D, Drachman D, et al, The genetic defect causing familial Alzheimer's disease maps on chromosome 21. Science235:885-90 1987 |
| PubMed ID: 2880399 |
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| Nee LE, Polinsky RJ, Eldridge R, Weingartner H, Smallberg S, Ebert M, A family with histologically confirmed Alzheimer's disease. Arch Neurol40:203-8 1983 |
| PubMed ID: 6600923 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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