AG08471
Fibroblast from Skin, Thorax/abdomen
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Thorax/abdomen
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thorax/abdomen
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
24 |
| PDL at Freeze |
3 |
| Passage Frozen |
1 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The biopsy was taken 7 hr post-mortem on 10/24/85. Cause of death was thromboembolus in left internal carotid and middle cerebral arteries. Donor had a 60 pack/year history of smoking and tuberculosis at age 3-4. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Frankel D, Delecourt V, Novoa-Del-Toro EM, Robin JD, Airault C, Bartoli C, Carabalona A, Perrin S, Mazaleyrat K, De Sandre-Giovannoli A, Magdinier F, Baudot A, Lévy N, Kaspi E, Roll P, miR-376a-3p and miR-376b-3p overexpression in Hutchinson-Gilford progeria fibroblasts inhibits cell proliferation and induces premature senescence iScience25:103757 2021 |
| PubMed ID: 35118365 |
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| El-Bazzal L, Rihan K, Bernard-Marissal N, Castro C, Chouery-Khoury E, Desvignes JP, Atkinson A, Bertaux K, Koussa S, Lévy N, Bartoli M, Mégarbané A, Jabbour R, Delague V, Loss of Cajal bodies in motor neurons from patients with novel mutations in VRK1 Human molecular genetics28:2378-2394 2019 |
| PubMed ID: 31090908 |
| PDL at Senescence |
24 |
| Cumulative PDL at Freeze |
3 |
| Passage Frozen |
1 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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