AG08480
Smooth muscle from Aorta, thoracic
Description:
CANIS
ORDER: CARNIVORA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Canis familiaris
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Common Name
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dog
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Remarks
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| PDL at Senescence |
16 |
| PDL at Freeze |
5 |
| Passage Frozen |
1 |
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| Remarks |
Beagle breed. The thoracic aorta was removed post-mortem on 10/28/85. Strips of the intimal and medial layers of the vessel were removed, minced, and used as explants to initiate the culture. Cell morphology is fibroblast-like. The cell culture tested positive for alpha-smooth muscle actin, negative for the endothelial cell-specific von Willebrand factor, (immunofluorescence assays), and negative with Dil-Acylated-LDL assay. Other cultures from this dog are AG08478 and AG08479. |
| PDL at Senescence |
16 |
| Cumulative PDL at Freeze |
5 |
| Passage Frozen |
1 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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