AG08493
Fibroblast from Skin, Arm
Description:
MACACA NEMESTRINA
ORDER: PRIMATES
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Nonhuman Primates
Animals |
|
Biopsy Source
|
Arm
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Arm
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
42,XY
|
|
Species
|
Macaca nemestrina
|
|
Common Name
|
pigtailed macaque
|
|
Remarks
|
|
| PDL at Senescence |
10 |
| PDL at Freeze |
4 |
| Passage Frozen |
3 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Genbank |
COX1 |
| Remarks |
The skin biopsy was taken ante-mortem on 10/30/85 from the inner aspect of the upper left arm. |
| PDL at Senescence |
10 |
| Cumulative PDL at Freeze |
4 |
| Passage Frozen |
3 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|