AG08501
Smooth muscle from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
82 |
| PDL at Freeze |
3 |
| Passage Frozen |
1 |
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| Remarks |
Hereford breed. The age of the donor is estimated. The thoracic aorta was removed immediately post-mortem on 10/22/85. Strips of the medial layer were removed, minced, and used as explants to initiate the culture. The cell morphology is fibroblast-like. The culture tested positive for alphasmooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluoresence assays). |
| PDL at Senescence |
82 |
| Cumulative PDL at Freeze |
3 |
| Passage Frozen |
1 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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