AG08522
LCL from B-Lymphocyte
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
German Alzheimer Disease |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Ethnicity
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GERMAN
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Family Member
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39
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Relation to Proband
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VI-19
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,t(5;17)(5pter>5q35::17q21>17qter; 17pter>17q21::5q35>5qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The donor does not admit to any problems with memory. His family has noticed a progressive change in personality as he has also manifested increasing difficulty with recent memory. His neurological exam was remarkable only for poor recent memory. The patient has refused further neurological evaluation. The culture was initiated on 11/05/85 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. The karyotype is 46,XY,t(5;17)(5pter>5q35:: 17q21>17qter;17pter>17q21::5q35>5qter). A skin fibroblast culture from same donor is AG08523. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Frommelt P, Schnabel R, Kuhne W, Nee LE, Polinsky RJ, Familial Alzheimer disease: a large, multigeneration German kindred. Alzheimer Dis Assoc Disord5:36-43 1991 |
| PubMed ID: 2025423 |
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| St George-Hyslop PH, Tanzi RE, Polinsky RJ, Haines JL, Nee L, Watkins PC, Myers RH, Feldman RG, Pollen D, Drachman D, et al, The genetic defect causing familial Alzheimer's disease maps on chromosome 21. Science235:885-90 1987 |
| PubMed ID: 2880399 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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