AG09389
LCL from B-Lymphocyte
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Family Member
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5
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Relation to Proband
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brother
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The donor is the normal brother of a Down's syndrome child. The culture was initiated on 2/17/87 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. The karyotype is 46,XY; normal diploid male. The affected brother of this donor is AG09394. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| San Roman AK, Godfrey AK, Skaletsky H, Bellott DW, Groff AF, Harris HL, Blanton LV, Hughes JF, Brown L, Phou S, Buscetta A, Kruszka P, Banks N, Dutra A, Pak E, Lasutschinkow PC, Keen C, Davis SM, Tartaglia NR, Samango-Sprouse C, Muenke M, Page DC, The human inactive X chromosome modulates expression of the active X chromosome Cell genomics3:100259 2022 |
| PubMed ID: 36819663 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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