AG09872
Endothelial from Artery, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Artery, iliac
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Artery, iliac
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Race
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Black/African American
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,dup(11)(pter>p15.1::p15.5>qter)[23]/46,XY[27]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
21 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line HIAE-43/7-12. The iliac artery was removed from a braindead, heartbeating cadaver, renal donor. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. The culture is a mosaic with karyotype: 46,XY/47,XY,+11/ 92,XXYY; unbalanced; 84%/6%/10%. This culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alphasmooth muscle actin (immunofluorescence assays). The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Admasu TD, Kim K, Rae M, Avelar R, Gonciarz RL, Rebbaa A, Pedro de Magalhães J, Renslo AR, Stolzing A, Sharma A, Selective ablation of primary and paracrine senescent cells by targeting iron dyshomeostasis Cell reports42:112058 2022 |
| PubMed ID: 36753419 |
| Cumulative PDL at Freeze |
21 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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