AG10220
Smooth muscle from Trachealis muscle
Description:
ORYCTOLAGUS
ORDER: LAGOMORPHA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Trachealis muscle
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Cell Type
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Smooth muscle
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Tissue Type
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Muscle
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Trachealis muscle
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Oryctolagus cuniculus
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Common Name
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rabbit
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Remarks
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| PDL at Senescence |
127 |
| PDL at Freeze |
25 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Clone LTSMC-3Q. This culture is a clonal derivative of line LTSMC-3, AG10187, from a 6 month old male New Zealand White rabbit, initiated on 7/14/88 using cells released by protease/collagenase digestion. Cell morphology is epithelial-like. In vitro lifespan studies on this culture were halted at PDL 127. This culture may be immortal. The culture tested positive for alpha-smooth muscle actin (immunofluorescence assay). |
| PDL at Senescence |
127 |
| Cumulative PDL at Freeze |
25 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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