AG10553
Fibroblast from Skin, Thorax/abdomen
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Thorax/abdomen
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thorax/abdomen
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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60,XY
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
36 |
| PDL at Freeze |
7 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
Holstein breed. The skin biopsy was taken 30 minutes post-mortem on 6/07/89 from the thoracic/abdominal area. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. The karyotype is 60,XY; normal diploid male. Other cultures from this calf are AG10554 and AG10604A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Murphy WJ, Fronicke L, O'Brien SJ, Stanyon R, The origin of human chromosome 1 and its homologs in placental mammals. Genome Res13(8):1880-8 2003 |
| PubMed ID: 12869576 |
| PDL at Senescence |
36 |
| Cumulative PDL at Freeze |
7 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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