AG10821
LCL from B-Lymphocyte
Description:
ALZHEIMER DISEASE; AD
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Alzheimer's Disease |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
|
|
Race
|
White
|
|
Ethnicity
|
ITALIAN
|
|
Family Member
|
4
|
|
Relation to Proband
|
brother
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
The donor is 50% at risk for Alzheimer's disease. Donor's deceased mother and 2 maternal uncles were affected. The culture was initiated on 1/23/90 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. Donor's father is AG10824 and his siblings are AG10825B, AG10822, and AG10823A. |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|