AG12372
Smooth muscle from Aorta, thoracic
Description:
SUS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aorta, thoracic
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Ethnicity
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YORKSHIRE
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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38,XX
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Species
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Sus scrofa
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Common Name
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pig
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Remarks
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| PDL at Senescence |
62 |
| PDL at Freeze |
16 |
| Passage Frozen |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Yorkshire breed. The thoracic aorta was removed immediately post-mortem. Strips of the medial layer were removed, minced, and used as explants to initiate the culture. Cell morphology is fibroblast-like. The culture tested positive for alpha-smooth muscle actin, negative for the endothelial cell-specific von Willebrand factor (immunofluoresence assays), and negative with Dil-acylated-LDL assay. Culture medium: HF12 15U. The HF12 medium used should be endotoxin-free. For lifespan determination this cell line was cultured on gelatin-coated flasks. The karyotype is 38,XX with 6% of the cells examined showing random chromosome loss/gain. Other cultures from this pig are AG12362, AG12363, and AG12417. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| PDL at Senescence |
62 |
| Cumulative PDL at Freeze |
16 |
| Passage Frozen |
8 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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