AG14615
LCL from B-Lymphocyte
Description:
SARDINIAN POPULATION OF GREAT AGE
APPARENTLY HEALTHY INDIVIDUAL
NIA AGING CELL REPOSITORY DNA PANEL - LONGEVITY COLLECTION
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Ethnicity
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ITALIAN
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Healthy nonagenarian from Carloforte, a city on San Pietro, an island off the southwest coast of Sardinia, and from a population very largely originating in Genoa in the 18th century. This individual is not related to the other members of the Carloforte collection for two generations.The culture was initiated on 7/7/97 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. The karyotype is 46,XY with 2% of the cells examined showing random chromosome loss. Follow up exam found donor subject alive and healthy at age 98. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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