AG14745
LCL from B-Lymphocyte
Description:
SARDINIAN POPULATION OF GREAT AGE
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Ethnicity
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ITALIAN
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY[92]/47,XY,+12[8]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Healthy octogenarian from Carloforte, a city on San Pietro, an island off the southwest coast of Sardinia, and from a population very largely originating in Genoa in the 18th century. This individual was not related to the other members of the Carloforte collection for two generations and lived to be 86 years of age.The culture was initiated on 9/30/97 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. The culture is a mosaic with karyotype: 46,XY[92]/47,XY,+12[8] with 2% of the cells examined showing random chromosome loss. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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