AG16896

Overview

Repository

NIA Aging Cell Culture Repository

Subcollection

Apparently Healthy Collection

Biopsy Source

Peripheral vein

Cell Type

B-Lymphocyte

Tissue Type

Blood

Transformant

Epstein-Barr Virus

Sample Source

LCL from B-Lymphocyte

Race

White

Ethnicity

IRISH/DUTCH

Relation to Proband

proband

Confirmation

Clinical summary/Case history

ISCN

46,XX

Species

Homo sapiens

Common Name

Human

Remarks

Donor had skin cancer of the face and has glaucoma, angina, osteoporosis, and polycythemia; family history of skin cancer and hypertension; mother lived to be over 90 years of age. The culture was initiated on 7/18/01 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical.The karyotype is 46,XX with 6% of the cells examined showing random chromosome loss/gain. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Characterizations

IDENTIFICATION OF SPECIES OF ORIGIN

Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis

External Links

dbSNP

dbSNP ID: 18238

Images

View

karyotype

Culture Protocols

Split Ratio

1:3

Temperature

37 C

Percent CO2

Medium

Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent

Serum

15% fetal bovine serum Not Inactivated

Substrate

None specified

Subcultivation Method

dilution - add fresh medium

Supplement

Description:

NIA AGING CELL REPOSITORY'S LONGEVITY RESEARCH PROGRAM

Affected:

No

Sex:

Female

Age:

96 YR (At Sampling)

Overview

Characterizations

Phenotypic Data

External Links

Images

Culture Protocols