AG19289
Fibroblast from Skin, Leg
Description:
NON WOUND HEALING PHENOTYPE
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Non-Healing Wound Collection |
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Biopsy Source
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Leg
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Leg
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Culture established from cells from non-healing calf wound complicated by infection; donor subject has on her left calf an upper anterior wound and a lower posterior wound; tissue was debrided from level two (deeper subcutaneous tissue) of the posterior wound; donor has type II diabetes; donor has calcified arteries in the limb with only mild peripheral arterial disease; venous reflux ulcers confirmed by ultrasound; on prednisone (10 mg/day) and intravenous ancef |
| Brem H, Golinko MS, Stojadinovic O, Kodra A, Diegelmann RF, Vukelic S, Entero H, Coppock DL, Tomic-Canic M, Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF Journal of translational medicine6:75 2008 |
| PubMed ID: 19046453 |
| Cumulative PDL at Freeze |
5 |
| Passage Frozen |
3 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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