AG21232
Adipose stromal cell from Skin, Unspecified
Description:
RATTUS
ORDER: RODENTIA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Unspecified
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Cell Type
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Adipose stromal cell
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Tissue Type
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Fat
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Transformant
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Spontaneous Transformation
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Sample Source
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Adipose stromal cell from Skin, Unspecified
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Rattus norvegicus
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Common Name
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brown or Norway rat
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Remarks
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| PDL at Senescence |
28 |
| PDL at Freeze |
4.3 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Adipose stromal cell culture made from fat from a Fisher F344 rat |
| PDL at Senescence |
28 |
| Cumulative PDL at Freeze |
7.28 |
| Passage Frozen |
3 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Adipose Stromal Cell Medium |
| Serum |
0% fetal bovine serum Not inactivated |
| Substrate |
Human Fibronectin |
| Supplement |
- |
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