AG22177
Fibroblast from Skin, Leg
Description:
NON WOUND HEALING PHENOTYPE
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Non-Healing Wound Collection |
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Biopsy Source
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Leg
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Leg
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Race
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Black/African American
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Ethnicity
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AFRICAN-AMERICAN
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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47,XXX[5]/46,XX[45]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
27 |
| PDL at Freeze |
5.65 |
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Clinically affected; ex-smoker; valvular disease; hypertriglyceridemia; anxiety; hypertension; hysterectomy; left lower pretibial region with ulcer (15 x 15cm with fibrinous exudate); ulcer is shallow with no pus or erythema; exposed tendon and tenderness; culture established from explants of skin tissue removed at the time of wound debridement and skin grafting |
| Brem H, Golinko MS, Stojadinovic O, Kodra A, Diegelmann RF, Vukelic S, Entero H, Coppock DL, Tomic-Canic M, Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF Journal of translational medicine6:75 2008 |
| PubMed ID: 19046453 |
| PDL at Senescence |
27 |
| Cumulative PDL at Freeze |
5.65 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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