AG23071
Fibroblast from Skin, Ear
Description:
AMES DWARF MOUSE PROJECT
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals
Ames Dwarf Mice |
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Biopsy Source
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Ear
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Ear
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Family Member
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4
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Family History
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N
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Relation to Proband
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littermate
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| PDL at Freeze |
3.2 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Ames normal-sized control mouse; this mutant is heterozygous for a recessive mutation in the Prop1 gene [Prop1+/Prop1(df)]; skin fibroblast culture was established from minced ear tissue taken at time of euthanasia. |
| Cumulative PDL at Freeze |
3.2 |
| Passage Frozen |
2 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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