AG23121
Fibroblast from Skin, Ear
Description:
AMES DWARF MOUSE PROJECT
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals
Ames Dwarf Mice |
|
Biopsy Source
|
Ear
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Ear
|
|
Family Member
|
5
|
|
Family History
|
N
|
|
Relation to Proband
|
littermate
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Mus musculus
|
|
Common Name
|
house mouse
|
|
Remarks
|
|
| PDL at Freeze |
3.08 |
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Ames normal-sized control mouse; this mutant is heterozygous for a recessive mutation in the Prop1 gene [Prop1+/Prop1(df)]; skin fibroblast culture was established from minced ear tissue taken at time of euthanasia |
| Cumulative PDL at Freeze |
3.08 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
|
|