AG23144
Adipose stromal cell from Skin, Unspecified
Description:
AMES DWARF MOUSE PROJECT
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Animals Ames Dwarf Mice |
Biopsy Source
|
Unspecified
|
Cell Type
|
Adipose stromal cell
|
Tissue Type
|
Fat
|
Transformant
|
Untransformed
|
Sample Source
|
Adipose stromal cell from Skin, Unspecified
|
Family Member
|
4
|
Family History
|
N
|
Relation to Proband
|
sister
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
40,XX
|
Species
|
Mus musculus
|
Common Name
|
house mouse
|
Remarks
|
|
PDL at Senescence |
26 |
PDL at Freeze |
1.18 |
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Remarks |
Ames normal-sized control mouse; this mutant is heterozygous for a recessive mutation in the Prop1 gene [Prop1df/WT]; adipose stromal culture was established from fat tissue taken at time of euthanasia. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN |
PDL at Senescence |
26 |
Cumulative PDL at Freeze |
3.95 |
Passage Frozen |
2 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
3% |
Medium |
Adipose Stromal Cell Medium |
Serum |
88% fetal bovine serum Not inactivated |
Substrate |
Human Fibronectin |
Supplement |
- |
|
|