Description:
TRANSLOCATED CHROMOSOME
CHROMOSOME DELETION
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XY,der(7)(7qter>7p21.2::10q11.21>10qter),der(10)(10pter>10q11.21::7p22.1>7pter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
5 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Congenital malformations; severe retardation; see GM00633 Lymphoid; deletion of 7p21.2p22.1 |
| Vortkamp A, Thias U, Gessler M, Rosenkranz W, Kroisel PM, Tommerup N, Kruger G, Gotz J, Pelz L, Grzeschik KH, A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p. Genomics11:737-43 1991 |
| PubMed ID: 1663489 |
| |
| Lebo RV, Golbus MS, Cheung MC, Detecting abnormal human chromosome constitutions by dual laser flow cytogenetics. Am J Med Genet25:519-29 1986 |
| PubMed ID: 2431619 |
| |
| Miller RC, Bradt C, Nichols WW, Mao TS, Yolken NY, Coriell LL, Greene AE, A (7;10) translocation, balanced, 46 chromosomes. Repository identification No. GM-44. Cytogenet Cell Genet14:86-7 1975 |
| PubMed ID: 1132253 |
| Passage Frozen |
5 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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