GM00118
Fibroblast from Uterine muscle, Uterus
Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Uterus
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Cell Type
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Fibroblast
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Tissue Type
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Uterine muscle
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Uterine muscle, Uterus
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Race
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White
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Ethnicity
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DANISH
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XX,der(15)(15qter>15p1:: Yq11>Yqter)pat
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Cytogenetics |
Chromosome 15: DERIVATIVE CHROMOSOME Aneuploid Segment (-)15pter>15p11 |
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Chromosome Y: DERIVATIVE CHROMOSOME Aneuploid Segment (+)Yq11>Yqter |
| Remarks |
Uterine tissue fibroblast culture; phenotypically normal |
| Herzing LB, Cook Jr EH Jr, Ledbetter DH, Allele-specific expression analysis by RNA-FISH demonstrates preferential maternal expression of UBE3A and imprint maintenance within 15q11- q13 duplications. Hum Mol Genet11(15):1707-18 2002 |
| PubMed ID: 12095913 |
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| Lin MS, Zhang A, Fujimoto A, Asynchronous DNA replication between 15q11.2q12 homologs: cytogenetic evidence for maternal imprinting and delayed replication. Hum Genet96(5):572-6 1995 |
| PubMed ID: 8530005 |
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| Tyler-Smith C, Oakey RJ, Larin Z, Fisher RB, Crocker M, Affara NA, Ferguson-Smith MA, Muenke M, Zuffardi O, Jobling MA, Localization of DNA sequences required for human centromere function through an analysis of rearranged Y chromosomes. Nat Genet5:368-75 1993 |
| PubMed ID: 8298645 |
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| Su TS, Nussbaum RL, Airhart S, Ledbetter DH, Mohandas T, O'Brien WE, Beaudet AL, Human chromosomal assignments for 14 argininosuccinate synthetase pseudogenes: cloned DNAs as reagents for cytogenetic analysis. Am J Hum Genet36:954-64 1984 |
| PubMed ID: 6093508 |
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| Hahnenmann N, Miller RC, Greene AE, Coriell LL, A (Y;15) translocation, 46 chromosomes. Repository identification No. GM-118. Cytogenet Cell Genet15:408-409 1975 |
| PubMed ID: 1225503 |
| Passage Frozen |
5 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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