Description:
ANEUPLOID CHROMOSOME NUMBER - NON-TRISOMIC
DICENTRIC CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,X,idic(X)(qter>p22::p22>qter)[35]/45,X[15]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
16 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Cytogenetics |
Chromosome X: ANEUPLOID Aneuploid Segment (+)Xp22>Xqter |
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Chromosome X: ANEUPLOID Aneuploid Segment (-)Xpter>Xp22 |
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Chromosome X: ANEUPLOID Trisomic Segment (+)Xp22>Xqter |
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Chromosome X: DICENTRIC CHROMOSOME Aneuploid Segment (+)Xp22>Xqter |
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Chromosome X: DICENTRIC CHROMOSOME Aneuploid Segment (-)Xpter>Xp22 |
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Chromosome X: DICENTRIC CHROMOSOME Trisomic Segment (+)Xp22>Xqter |
| Remarks |
Ovarian dysgenesis without Turner stigmata; 70% of cells have the dicentric chromosome; dicentric X is late replicating |
| Carrel L, Willard HF, Heterogeneous gene expression from the inactive X chromosome: an X-linked gene that escapes X inactivation in some human cell lines but is inactivated in others. Proc Natl Acad Sci U S A96:7364-9 1999 |
| PubMed ID: 10377420 |
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| Higgins AW, Schueler MG, Willard HF, Chromosome engineering: generation of mono- and dicentric isochromosomes in a somatic cell hybrid system. Chromosoma108:256-65 1999 |
| PubMed ID: 10460414 |
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| Su TS, Nussbaum RL, Airhart S, Ledbetter DH, Mohandas T, O'Brien WE, Beaudet AL, Human chromosomal assignments for 14 argininosuccinate synthetase pseudogenes: cloned DNAs as reagents for cytogenetic analysis. Am J Hum Genet36:954-64 1984 |
| PubMed ID: 6093508 |
| Passage Frozen |
16 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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