GM00970
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
7.58 |
| Passage Frozen |
7 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Skin biopsy; 46,XY; 6% of cells show random chromosome loss |
| Cappelli E, D'Osualdo A, Bogliolo M, Kelley MR, Frosina G, Drosophila S3 ribosomal protein accelerates repair of 8-oxoguanine performed by human and mouse cell extracts Environmental and molecular mutagenesis42:50-8 2003 |
| PubMed ID: 12874813 |
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| Kim BE, Smith K, Petris MJ, A copper treatable Menkes disease mutation associated with defective trafficking
of a functional Menkes copper ATPase. J Med Genet40(4):290-5 2003 |
| PubMed ID: 12676902 |
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| Hartig R, Shoeman RL, Janetzko A, Grub S, Traub P, Active nuclear import of single-stranded oligonucleotides and their complexes with non-karyophilic macromolecules. Biol Cell90(5):407-26 1998 |
| PubMed ID: 9835015 |
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| Hartig R, Shoeman RL, Janetzko A, Tolstonog G, Traub P, DNA-mediated transport of the intermediate filament protein vimentin into the nucleus of cultured cells. J Cell Sci111 ( Pt 24):3573-84 1998 |
| PubMed ID: 9819349 |
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| Rodriguez A, Webster P, Ortego J, Andrews NW, Lysosomes behave as Ca2+-regulated exocytic vesicles in fibroblasts and epithelial cells. J Cell Biol137(1):93-104 1997 |
| PubMed ID: 9105039 |
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| Weiss MJ, Ray K, Fallon MD, Whyte MP, Fedde KN, Lafferty MA, Mulivor RA, Harris H, Analysis of liver/bone/kidney alkaline phosphatase mRNA, DNA, and enzymatic activity in cultured skin fibroblasts from 14 unrelated patients with severe hypophosphatasia. Am J Hum Genet44:686-94 1989 |
| PubMed ID: 2705456 |
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| Butler JD, Key JD, Hughes BF, Tietze F, Raiford DS, Reed GF, Brannon PM, Spielberg SP, Schulman JD, Glutathione metabolism in normal and cystinotic fibroblasts. Exp Cell Res172:158-67 1987 |
| PubMed ID: 2888673 |
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| Shaham M, Adler B, Ganguly S, Chaganti RS, Transfection of normal human and Chinese hamster DNA corrects diepoxybutane-induced chromosomal hypersensitivity of Fanconi anemia fibroblasts. Proc Natl Acad Sci U S A84:5853-7 1987 |
| PubMed ID: 3475705 |
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| Barbis DP, Schultz RA, Friedberg EC, Isolation and partial characterization of virus-transformed cell lines representing the A, G and variant complementation groups of xeroderma pigmentosum. Mutat Res165:175-84 1986 |
| PubMed ID: 3010096 |
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| de Wett W, Sippola M, Tromp G, Prockop D, Chu ML, Ramirez F, Use of R-loop mapping for the assessment of human collagen mutations. J Biol Chem261:3857-62 1986 |
| PubMed ID: 3949794 |
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| Sephel GC, Davidson JM, Elastin production in human skin fibroblast cultures and its decline with age. J Invest Dermatol86:279-85 1986 |
| PubMed ID: 3745952 |
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| Thompson JN, Roden L, Reynertson R, Oligosaccharide substrates for heparin sulfamidase. Anal Biochem152:412-22 1986 |
| PubMed ID: 3083713 |
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| Schultz RA, Barbis DP, Friedberg EC, Studies on gene transfer and reversion to UV resistance in xeroderma pigmentosum cells. Somat Cell Mol Genet11:617-24 1985 |
| PubMed ID: 3000003 |
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| Behrens UJ, Paronetto F, Elimination of the yeast Candida parapsilosis from lymphoid cells and monolayer cells in culture. In Vitro20:391-5 1984 |
| PubMed ID: 6586685 |
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| Ben-Ishai R, Sharon R, Rothman M, Miskin R, DNA repair and induction of plasminogen activator in human fetal cells treated with ultraviolet light. Carcinogenesis5:357-62 1984 |
| PubMed ID: 6538462 |
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| de Wet WJ, Chu ML, Prockop DJ, The mRNAs for the pro-alpha 1(I) and pro-alpha 2(I) chains of type I procollagen are translated at the same rate in normal human fibroblasts and in fibroblasts from two variants of osteogenesis imperfecta with altered steady state ratios of the two mRNAs. J Biol Chem258:14385-9 1983 |
| PubMed ID: 6689020 |
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| Murad S, Tajima S, Johnson GR, Sivarajah S, Pinnell SR, Collagen synthesis in cultured human skin fibroblasts: effect of ascorbic acid and its analogs. J Invest Dermatol81:158-62 1983 |
| PubMed ID: 6308103 |
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| Gitelman, Regional mapping of the loci for hexokinase-1(HK1) and glutamic oxaloacetic transaminase-1(GOT1) on chromosome 10 by gene dosage studies. Cytogenet Cell Genet32:281 (1982):158-62 1982 |
| PubMed ID: 6308103 |
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| Honey NK, Mueller OT, Little LE, Miller AL, Shows TB, Mucolipidosis III is genetically heterogeneous. Proc Natl Acad Sci U S A79:7420-4 1982 |
| PubMed ID: 6961420 |
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| Shows TB, Mueller OT, Honey NK, Wright CE, Miller AL, Genetic heterogeneity of I-cell disease is demonstrated by complementation of lysosomal enzyme processing mutants. Am J Med Genet12:343-53 1982 |
| PubMed ID: 6287841 |
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| Murad S, Grove D, Lindberg KA, Reynolds G, Sivarajah A, Pinnell SR, Regulation of collagen synthesis by ascorbic acid. Proc Natl Acad Sci U S A78:2879-82 1981 |
| PubMed ID: 6265920 |
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| Murad S, Sivarajah A, Pinnell SR, Regulation of prolyl and lysyl hydroxylase activities in cultured human skin fibroblasts by ascorbic acid. Biochem Biophys Res Commun101:868-75 1981 |
| PubMed ID: 6272771 |
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| Breslow JL, Epstein J, Vayo MM, Fontaine JH, Decreased ouabain binding in cystic fibrosis fibroblasts in potassium- free medium. Exp Cell Res110:399-407 1977 |
| PubMed ID: 590361 |
| Passage Frozen |
7 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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