Description:
TRIPLOID CHROMOSOME NUMBER
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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68,XX[1]/69,XXY[49]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
6 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Remarks |
Meningomyelocele; phenotypically female; G6PD type AB; both X chromosomes are active and early-replicating |
| Moyzis RK, Albright KL, Bartholdi MF, Cram LS, Deaven LL, Hildebrand CE, Joste NE, Longmire JL, Meyne J, Schwarzacher-Robinson T, Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis. Chromosoma95:375-86 1987 |
| PubMed ID: 3677921 |
| |
| Migeon BR, Wolf SF, Mareni C, Axelman J, Derepression with decreased expression of the G6PD locus on the inactive X chromosome in normal human cells. Cell29:595-600 1982 |
| PubMed ID: 7116451 |
| |
| Lin MS, Sister chromatid exchanges in human cells and Chinese hamster cells. Evidence that the rate of sister chromatid exchanges is a function of ploidy. Exp Cell Res127:179-83 1980 |
| PubMed ID: 7379862 |
| |
| Willard HF, Breg WR, Human X chromosomes: synchrony of DNA replication in diploid and triploid fibroblasts with multiple active or inactive X chromosomes. Somatic Cell Genet6:187-98 1980 |
| PubMed ID: 6156493 |
| |
| Migeon BR, Sprenkle JA, Do TT, Stability of the "two active X" phenotype in triploid somatic cells. Cell18:637-41 1979 |
| PubMed ID: 229959 |
| Passage Frozen |
6 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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