GM02803

GM02803 Fibroblast

Description:

CYSTIC FIBROSIS; CF

Affected:

No

Sex:

Male

Age:

27 YR (At Sampling)

Overview

Repository

NIGMS Human Genetic Cell Repository

Subcollection

Heritable Diseases

Class

Other Disorders of Known Biochemistry

Cell Type

Fibroblast

Transformant

Untransformed

Race

White

Family Member

Relation to Proband

brother

Confirmation

Clinical summary/Case history

Species

Homo sapiens

Common Name

Human

Remarks

Unaffected; possible heterozygote; normal sweat electrolytes; sister died at age 25 of CF

Characterizations

Passage Frozen

IDENTIFICATION OF SPECIES OF ORIGIN

Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis

Phenotypic Data

Remarks

Unaffected; possible heterozygote; normal sweat electrolytes; sister died at age 25 of CF

Publications

Colicos MA, Haj-Ahmad Y, Valerie K, Henderson EE, Rainbow AJ, Construction of a recombinant adenovirus containing the denV gene from bacteriophage T4 which can partially restore the DNA repair deficiency in xeroderma pigmentosum fibroblasts. Carcinogenesis12:249-55 1991

PubMed ID: 1704821

External Links

dbSNP

dbSNP ID: 20836

NCBI GTR

219700 CYSTIC FIBROSIS; CF

OMIM

219700 CYSTIC FIBROSIS; CF

Omim Description

CYSTIC FIBROSIS; CF

MUCOVISCIDOSIS

Images

View

pedigree

Culture Protocols

Passage Frozen

Split Ratio

1:5

Temperature

37 C

Percent CO2

Percent O2

AMBIENT

Medium

Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent

Serum

15% fetal bovine serum Not inactivated

Substrate

None specified

Subcultivation Method

trypsin-EDTA

Supplement

Pricing

Commercial/For-profit:

$311.00USD

Academic/Non-profit/Government:

$176.00USD