Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XY,t(15;18)(15pter>15q15::18p11.3> 18pter;18qter>18p11.3::15q15>15qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
5 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Cytogenetics |
Chromosome 15: TRANSLOCATION Breakpoint 15q15 t(15;18)15q15 |
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Chromosome 18: TRANSLOCATION Breakpoint 18p11 t(15;18)18p11 |
| Remarks |
Phenotypically normal; died 4 hours after birth |
| Markie D, Jones TA, Sheer D, Bodmer WF, A somatic cell hybrid panel for regional mapping of human chromosome 18. Genomics14:431-6 1992 |
| PubMed ID: 1427858 |
| |
| Mattei MG, Souiah N, Mattei JF, Chromosome 15 anomalies and the Prader-Willi syndrome: cytogenetic analysis. Hum Genet66:313-34 1984 |
| PubMed ID: 6373566 |
| Passage Frozen |
5 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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