GM05267
Kidney-derived cell line from Kidney
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Kidney
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Cell Type
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Kidney-derived cell line
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Tissue Type
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Kidney
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Transformant
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Simian Virus 40
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Sample Source
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Kidney-derived cell line from Kidney
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Confirmation
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Clinical summary/Case history
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ISCN
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Mouse; modal no. of 38 chromosomes; 84% of cells lack a Y chromosome
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
SV40 transformed tfm mouse kidney cells; HPRT deficient; selectable with 6-thioguanine; expresses testicular feminization locus mutation: little detectable androgen binding activity; modal number of 38 chromosomes |
| Jabs EW, Wolf SF, Migeon BR, Characterization of a cloned DNA sequence that is present at centromeres of all human autosomes and the X chromosome and shows polymorphic variation. Proc Natl Acad Sci U S A81:4884-8 1984 |
| PubMed ID: 6589633 |
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| Migeon BR, Brown TR, Axelman J, Migeon CJ, Studies of the locus for androgen receptor: localization on the human X chromosome and evidence for homology with the Tfm locus in the mouse. Proc Natl Acad Sci U S A78:6339-43 1981 |
| PubMed ID: 6947233 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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