Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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Black/African American
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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ISCN
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46,XY,t(3;11)(p21;q23)[2]/46,XY[17]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4.24 |
| Passage Frozen |
6 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Remarks |
Lymphocytes show trisomic codominant expression of HLA A (34,19, 23) and B (13, 12,35) haplotypes; 46,XY in lymphocytes; in fibroblasts, 17% of cells have an additional t(3;11)(p21;q23) at passage 6 |
| Zhong Y, Yan W, Ruan J, Fang M, Lapidus RG, Du S, Fang S, A novel XBP1 variant is highly enriched in cancer tissues and is specifically required for cancer cell survival Biochemical and biophysical research communications562:69-75 2021 |
| PubMed ID: 34038755 |
| |
| Sarraf SA, Sideris DP, Giagtzoglou N, Ni L, Kankel MW, Sen A, Bochicchio LE, Huang CH, Nussenzweig SC, Worley SH, Morton PD, Artavanis-Tsakonas S, Youle RJ, Pickrell AM, PINK1/Parkin Influences Cell Cycle by Sequestering TBK1 at Damaged Mitochondria, Inhibiting Mitosis Cell reports29:225-235.e5 2018 |
| PubMed ID: 31577952 |
| |
| Goldman D, Giri PR, O'Brien SJ, A molecular phylogeny of the hominoid primates as indicated by two- dimensional protein electrophoresis. Proc Natl Acad Sci U S A84:3307-11 1987 |
| PubMed ID: 3106965 |
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| Morton, Trisomic codominant expression of major histocompatibility complex (MHC) due to meiotic recombination. 6th Int Cong Hum GenetAbstracts p 119 (1981):3307-11 1981 |
| PubMed ID: 3106965 |
| |
| Morton, Codominant expression of major histocompatibility complex (MHC) in a case of partial trisomy 6p resulting from an insertion and inversion involving heterologous chromosomes. Am J Hum Genet32:81A (1980):3307-11 1980 |
| PubMed ID: 3106965 |
| Passage Frozen |
6 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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