GM05386
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Skin biopsy; 14.0 cm crown-rump length |
| Yeh CJ, Chen CC, Leu YL, Lin MW, Chiu MM, Wang SH, The effects of artocarpin on wound healing: in vitro and in vivo studies Scientific reports7:15599 2017 |
| PubMed ID: 29142215 |
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| Zhou X, Cole SW, Rao NP, Cheng Z, Li Y, McBride J, Wong DT, Identification of discrete chromosomal deletion by binary recursive partitioning of microarray differential expression data Journal of medical genetics42:416-9 2005 |
| PubMed ID: 15863671 |
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| Zhou X, Cole SW, Hu S, Wong DT, Detection of DNA copy number abnormality by microarray expression analysis Human genetics114:464-7 2004 |
| PubMed ID: 14872338 |
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| Zhou X, Mok SC, Chen Z, Li Y, Wong DT, Concurrent analysis of loss of heterozygosity (LOH) and copy number abnormality (CNA) for oral premalignancy progression using the Affymetrix 10K SNP mapping array Human genetics115:327-30 2004 |
| PubMed ID: 15290239 |
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| Ovchinnikov I, Rubin A, Swergold GD, Tracing the LINEs of human evolution. Proc Natl Acad Sci U S A99(16):10522-7 2002 |
| PubMed ID: 12138175 |
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| Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH. J Biol Chem270:6243-5 1995 |
| PubMed ID: 7534307 |
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| Magnuson VL, Young M, Schattenberg DG, Mancini MA, Chen DL, Steffensen B, Klebe RJ, The alternative splicing of fibronectin pre-mRNA is altered during aging and in response to growth factors. J Biol Chem266:14654-62 1991 |
| PubMed ID: 1713586 |
| Passage Frozen |
4 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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