GM05387
Fibroblast from Lung, Lung
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Lung biopsy; 14.0 cm crown-rump length |
| Lamba DA, McUsic A, Hirata RK, Wang PR, Russell D, Reh TA, Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells PloS one5:e8763 2009 |
| PubMed ID: 20098701 |
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| Trobridge GD, Miller DG, Jacobs MA, Allen JM, Kiem HP, Kaul R, Russell DW, Foamy virus vector integration sites in normal human cells Proceedings of the National Academy of Sciences of the United States of America103:1498-503 2006 |
| PubMed ID: 16428288 |
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| Chen FW, Gordon RE, Ioannou YA, NPC1 late endosomes contain elevated levels of non-esterified ('free') fatty acids and an abnormally glycosylated form of the NPC2 protein The Biochemical journal390:549-61 2005 |
| PubMed ID: 15896196 |
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| Miller DG, Trobridge GD, Petek LM, Jacobs MA, Kaul R, Russell DW, Large-scale analysis of adeno-associated virus vector integration sites in normal human cells Journal of virology79:11434-42 2005 |
| PubMed ID: 16103194 |
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| Trobridge G, Hirata RK, Russell DW, Gene targeting by adeno-associated virus vectors is cell-cycle dependent Human gene therapy16:522-6 2005 |
| PubMed ID: 15871683 |
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| Trobridge G, Russell DW, Cell cycle requirements for transduction by foamy virus vectors compared to those of oncovirus and lentivirus vectors. J Virol78(5):2327-35 2004 |
| PubMed ID: 14963129 |
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| Greco M, Villani G, Mazzucchelli F, Bresolin N, Papa S, Attardi G, Marked aging-related decline in efficiency of oxidative phosphorylation in human skin fibroblasts The FASEB journal : official publication of the Federation of American Societies for Experimental Biology17:1706-8 2003 |
| PubMed ID: 12958183 |
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| Miller DG, Petek LM, Russell DW, Human gene targeting by adeno-associated virus vectors is enhanced by DNA
double-strand breaks. Mol Cell Biol23(10):3550-7 2003 |
| PubMed ID: 12724413 |
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| Walter M, Davies JP, Ioannou YA, Telomerase immortalization upregulates Rab9 expression and restores LDL cholesterol egress from Niemann-Pick C1 late endosomes. J Lipid Res44(2):243-53 2003 |
| PubMed ID: 12576506 |
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| Hirata RK, Russell DW, Design and packaging of adeno-associated virus gene targeting vectors Journal of virology74:4612-20 2000 |
| PubMed ID: 10775597 |
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| Leland Booth J, Metcalf JP, Type-specific induction of interleukin-8 by adenovirus. Am J Respir Cell Mol Biol21:521-7 1999 |
| PubMed ID: 10502562 |
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| Weekes MP, Wills MR, Mynard K, Carmichael AJ, Sissons JG, The memory cytotoxic T-lymphocyte (CTL) response to human cytomegalovirus infection contains individual peptide-specific CTL clones that have undergone extensive expansion in vivo. J Virol73:2099-108 1999 |
| PubMed ID: 9971792 |
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| Wills MR, Carmichael AJ, Weekes MP, Mynard K, Okecha G, Hicks R, Sissons JG, Human virus-specific CD8+ CTL clones revert from CD45ROhigh to CD45RAhigh in vivo: CD45RAhighCD8+ T cells comprise both naive and memory cells. J Immunol162(12):7080-7 1999 |
| PubMed ID: 10358151 |
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| Russell DW, Hirata RK, Human gene targeting by viral vectors [see comments] Nat Genet18:325-30 1998 |
| PubMed ID: 9537413 |
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| Rutledge EA, Halbert CL, Russell DW, Infectious clones and vectors derived from adeno-associated virus (AAV) serotypes other than AAV type 2. J Virol72:309-19 1998 |
| PubMed ID: 9420229 |
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| Bottles KD, Laszik Z, Morrissey JH, Kinasewitz GT, Tissue factor expression in mesothelial cells: induction both in vivo and in vitro. Am J Respir Cell Mol Biol17:164-72 1997 |
| PubMed ID: 9271304 |
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| Laderman KA, Penny JR, Mazzucchelli F, Bresolin N, Scarlato G, Attardi G, Aging-dependent functional alterations of mitochondrial DNA (mtDNA) from human fibroblasts transferred into mtDNA-less cells. J Biol Chem271(27):15891-7 1996 |
| PubMed ID: 8663253 |
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| Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH. J Biol Chem270:6243-5 1995 |
| PubMed ID: 7534307 |
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| Hershberger RP, Culp LA, Cell-type-specific expression of alternatively spliced human fibronectin IIICS mRNAs. Mol Cell Biol10:662-71 1990 |
| PubMed ID: 1688996 |
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| Bloem LJ, Chen L, Konigsberg WH, Bach R, Serum stimulation of quiescent human fibroblasts induces the synthesis of tissue factor mRNA followed by the appearance of tissue factor antigen and procoagulant activity. J Cell Physiol139:418-23 1989 |
| PubMed ID: 2715194 |
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| Bloem LJ, Chen L, Konigsberg WH, Bach R, Serum stimulation of quiescent human fibroblasts induces the synthesis of tissue factor mRNA followed by the appearance of tissue factor antigen and procoagulant activity. J Cell Physiol139:418-23 1989 |
| PubMed ID: 30122154 |
| Passage Frozen |
3 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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