GM05389
Fibroblast from Lung, Lung
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Race
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White
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Family Member
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2
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Relation to Proband
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twin
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
1 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Lung biopsy; 13.5 cm crown-rump length |
| Gotoh E, Visualizing Active Replication Regions in S-Phase Chromosomes Methods in molecular biology (Clifton, NJ)2519:117-126 2022 |
| PubMed ID: 36066717 |
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| Kucich U, Rosenbloom JC, Abrams WR, Rosenbloom J, Transforming growth factor-beta stabilizes elastin mRNA by a pathway requiring active Smads, protein kinase C-delta, and p38. Am J Respir Cell Mol Biol26(2):183-8 2002 |
| PubMed ID: 11804868 |
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| Kucich U, Rosenbloom JC, Abrams WR, Bashir MM, Rosenbloom J, Stabilization of elastin mRNA by TGF-beta: initial characterization of signaling pathway [see comments] Am J Respir Cell Mol Biol17:10-6 1997 |
| PubMed ID: 9224204 |
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| Scanlan MJ, Raj BK, Calvo B, Garin-Chesa P, Sanz-Moncasi MP, Healey JH, Old LJ, Rettig WJ, Molecular cloning of fibroblast activation protein alpha, a member of the serine protease family selectively expressed in stromal fibroblasts of epithelial cancers. Proc Natl Acad Sci U S A91:5657-61 1994 |
| PubMed ID: 7911242 |
| Passage Frozen |
1 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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