GM05420
Fibroblast from Conjunctiva, Conjunctiva
Description:
APPARENTLY HEALTHY FETAL TISSUE
MONOZYGOTIC TWIN PAIRS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Conjunctiva
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Cell Type
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Fibroblast
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Tissue Type
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Conjunctiva
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Conjunctiva, Conjunctiva
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Race
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White
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Family Member
|
2
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Relation to Proband
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twin brother
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Confirmation
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Karyotypic analysis and Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Conjunctiva biopsy; 13.5 cm crown-rump length; 46,XY; same patient as GM05388 and 05389; monozygotic twin of GM05421 |
| Paras Garg, Ricky S. Joshi, Corey Watson, Andrew J. Sharp, A survey of inter-individual variation in DNA methylation identifies environmentally responsive co-regulated networks of epigenetic variation in the human genome PLoS Genetics14: 2018 |
| PubMed ID: 30273333 |
| Passage Frozen |
3 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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