GM05758
Fibroblast from Skin, Inguinal area
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Inguinal area
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Inguinal area
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
7.11 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Skin biopsy (inguinal) |
| Fafián-Labora JA, O'Loghlen A, NF-?B/IKK activation by small extracellular vesicles within the SASP Aging cell20:e13426 2021 |
| PubMed ID: 34187082 |
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| Fafián-Labora JA, Rodríguez-Navarro JA, O'Loghlen A, Small Extracellular Vesicles Have GST Activity and Ameliorate Senescence-Related Tissue Damage Cell metabolism20:e13426 2020 |
| PubMed ID: 32574561 |
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| Naguib A, Sandmann T, Yi F, Watts RJ, Lewcock JW, Dowdle WE, SUPT4H1 Depletion Leads to a Global Reduction in RNA Cell reports26:45-53.e4 2018 |
| PubMed ID: 30605685 |
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| Karlsson KH, Stenerlow B, Focus formation of DNA repair proteins in normal and repair-deficient cells irradiated with high-LET ions. Radiat Res161(5):517-27 2004 |
| PubMed ID: 15161372 |
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| Radulescu I, Elmroth K, Stenerlow B, Chromatin organization contributes to non-randomly distributed double-strand breaks after exposure to high-LET radiation. Radiat Res161(1):1-8 2004 |
| PubMed ID: 14680402 |
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| Stenerlow B, Karlsson KH, Cooper B, Rydberg B, Measurement of prompt DNA double-strand breaks in mammalian cells without including heat-labile sites: results for cells deficient in nonhomologous end joining. Radiat Res159(4):502-10 2003 |
| PubMed ID: 12643795 |
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| Azzam EI, De Toledo SM, Spitz DR, Little JB, Oxidative metabolism modulates signal transduction and micronucleus formation in
bystander cells from alpha-particle-irradiated normal human fibroblast cultures. Cancer Res62(19):5436-42 2002 |
| PubMed ID: 12359750 |
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| Stenerlow B, Hoglund E, Rejoining of double-stranded DNA-fragments studied in different size-intervals. Int J Radiat Biol78(1):1-7 2002 |
| PubMed ID: 11747548 |
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| Hoglund H, Stenerlow B, Induction and rejoining of DNA double-strand breaks in normal human skin fibroblasts after exposure to radiation of different linear energy transfer: possible roles of track structure and chromatin organization. Radiat Res155(6):818-25 2001 |
| PubMed ID: 11352764 |
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| Hoglund E, Blomquist E, Carlsson J, Stenerlow B, DNA damage induced by radiation of different linear energy transfer: initial fragmentation. Int J Radiat Biol76(4):539-47 2000 |
| PubMed ID: 10815635 |
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| Stenerlow B, Hoglund E, Carlsson J, Blomquist E, Rejoining of DNA fragments produced by radiations of different linear energy transfer. Int J Radiat Biol76(4):549-57 2000 |
| PubMed ID: 10815636 |
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| Markello TC, Guo J, Gahl WA, High-performance liquid chromatography of lipids for the identification of human metabolic disease. Anal Biochem198:368-74 1991 |
| PubMed ID: 1799224 |
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| Weiss MJ, Ray K, Fallon MD, Whyte MP, Fedde KN, Lafferty MA, Mulivor RA, Harris H, Analysis of liver/bone/kidney alkaline phosphatase mRNA, DNA, and enzymatic activity in cultured skin fibroblasts from 14 unrelated patients with severe hypophosphatasia. Am J Hum Genet44:686-94 1989 |
| PubMed ID: 2705456 |
| Passage Frozen |
5 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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