Description:
INVERTED CHROMOSOME
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Chromosome Abnormalities |
Cell Type
|
Fibroblast
|
Transformant
|
Untransformed
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
ISCN
|
46,XX,inv(10)(pter>p11.2::q21>p11.2:: q21>qter)
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Passage Frozen |
4 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Cytogenetics |
Chromosome 10: INVERSION Breakpoint 10p11 inv(10)10p11 |
|
Chromosome 10: INVERSION Breakpoint 10q21 inv(10)10q21 |
Bailey SM, Meyne J, Cornforth MN, McConnell TS, Goodwin EH, A new method for detecting pericentric inversions using COD-FISH. Cytogenet Cell Genet75:248-53 1996 |
PubMed ID: 9067435 |
Passage Frozen |
4 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|