GM06166
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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Black/African American
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
9 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Skin biopsy; 46,XY; 2% of cells show random chromosome gain and 2% show random chromosomal aberrations; same patient as GM06167 |
| Storch TG, Talley GD, Oxygen concentration regulates the proliferative response of human fibroblasts to serum and growth factors. Exp Cell Res175:317-25 1988 |
| PubMed ID: 3258822 |
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| Wing DA, Talley GD, Storch TG, Oxygen concentration regulates EGF-induced proliferation and EGF- receptor down regulation. Biochem Biophys Res Commun153:952-8 1988 |
| PubMed ID: 3260495 |
| Passage Frozen |
9 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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