GM12511
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 17 SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Human/mouse hybrid retaining human del(17), #1, 2, 3, 5, 6, 10, 11, 12, 18, 19, 20, 21, & 22
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
Line RBCD1; produced by the fusion of human cells from a pt with isolated lissencephaly, 46,XY,del(17)(p13), with TKdeficient Cl1D mouse cells; grown in HAT medium; retains human del(17) in 56% of cells & other human chromosomes |
| Ledbetter SA, Kuwano A, Dobyns WB, Ledbetter DH, Microdeletions of chromosome 17p13 as a cause of isolated lissencephaly. Am J Hum Genet50:182-9 1992 |
| PubMed ID: 1346078 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
|
|