GM15993
LCL from B-Lymphocyte
Description:
TRANSLOCATED CHROMOSOME
BREAST CANCER
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
| Class |
Disorders of Uncertain Biochemical Etiology |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Family Member
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2
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Relation to Proband
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maternal aunt
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX,t(11;12)(11pter>11q24::12q22>12qter;12pter>12q22::11q24>11qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Cytogenetics |
Chromosome 11: TRANSLOCATION Breakpoint 11q24 t(11;12)11q24 |
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Chromosome 12: TRANSLOCATION Breakpoint 12q22 t(11;12)12q22 |
| Remarks |
Breast cancer diagnosed at age 42; niece also diagnosed with breast cancer at age 42 (GM15232) |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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