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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Genbank |
COX1 |
| Remarks |
This cell line was obtained from lung tissue of a young male Chinese hamster and was originally designated as V cells (Ford and Yerganian, 1958), was renamed V79 by Elkind in 1958, and widely used thereafter. Several different subclones subsequently appeared, such as V79-4 or V79B, and it is therefore of importance to use the true parental lines as controls for different mutants derived from different V79 cell lines. This V79 is the parental cell line for a series of repair deficient mutants, including V-B11 (GM16137), the only representative of complementation group 7 of UV-sensitive rodent cell mutants, V-C4 (GM16138) and V-E5 (GM16139) members of complementation group 8 of x-ray-sensitive rodent cell mutants. |
| Chesner LN, Essawy M, Warner C, Campbell C, DNA-protein crosslinks are repaired via homologous recombination in mammalian mitochondria DNA repair97:103026 2020 |
| PubMed ID: 33316746 |
| |
| Gottesman, M. M., "Molecular Cell Genetics", New York, Wiley DNA repair97:66-74 1985 |
| PubMed ID: 33316746 |
| Split Ratio |
1:14 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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