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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Chinese hamster lung mutant cell line V-H1, derived from V79 (GM16136) by ethylnitrosourea-induced mutagenesis. V-H1 is UV hypersensitive with reduced excision repair of photoproducts and belongs to complementation group 2 of UV-sensitive rodent mutants. V-H1 is mutated in the XPD/ERCC2 gene. |
| Kadkhodayan S, Salazar EP, Ramsey MJ, Takayama K, Zdzienicka MZ, Tucker JD,
Weber CA, Molecular analysis of ERCC2 mutations in the repair deficient hamster mutants
UVL-1 and V-H1. Mutat Res385(1):47-57 1997 |
| PubMed ID: 9372848 |
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| Zdzienicka MZ, Venema J, Mitchell DL, van Hoffen A, van Zeeland AA, Vrieling
H, Mullenders LH, Lohman PH, Simons JW, (6-4) photoproducts and not cyclobutane pyrimidine dimers are the main
UV-induced mutagenic lesions in Chinese hamster cells. Mutat Res273(1):73-83 1992 |
| PubMed ID: 1376437 |
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| Regan JD, Thompson LH, Carrier WL, Weber CA, Francis AA, Zdzienicka MZ, Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect
of the human ERCC2 repair gene. Mutat Res235(3):157-63 1990 |
| PubMed ID: 2342503 |
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| Mitchell DL, Zdzienicka MZ, van Zeeland AA, Nairn RS, Intermediate (6-4) photoproduct repair in Chinese hamster V79 mutant V-H1
correlates with intermediate levels of DNA incision and repair replication. Mutat Res226(1):43-7 1989 |
| PubMed ID: 2716768 |
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| Zdzienicka MZ, van der Schans GP, Westerveld A, van Zeeland AA, Simons JW, Phenotypic heterogeneity within the first complementation group of UV-sensitive
mutants of Chinese hamster cell lines. Mutat Res193(1):31-41 1988 |
| PubMed ID: 3336370 |
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| Zdzienicka MZ, Simons JW, Mutagen-sensitive cell lines are obtained with a high frequency in V79 Chinese
hamster cells. Mutat Res178(2):235-44 1987 |
| PubMed ID: 3587254 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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