GM29054

Overview

Repository

NIGMS Human Genetic Cell Repository

Subcollection

Gene-Edited hiPSC
Heritable Diseases
PIGI Consented Sample

Protocols

Protocol PDF

Biopsy Source

Skin

Cell Type

Stem cell

Cell Subtype

Induced pluripotent stem cell

Transformant

Reprogrammed (Retroviral)

Sample Source

iPSC from Fibroblast

Race

White

Ethnicity

Not Hispanic/Latino

Ethnicity

Irish, English, German, Dutch

Country of Origin

GERMANY

Family Member

Family History

Relation to Proband

proband

ISCN

46,XX[20]

Species

Homo sapiens

Common Name

Human

Remarks

See Phenotypic Data tab. This line is the isogenic control for the patient-derived iPSC line GM27896; parental fibroblast GM27897. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia, Japan Inc..

Characterizations

Passage Frozen

Induced Pluripotent Stem Cell

After mutation correction with CRISPR/Cas9 the cell line was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.