I91L-07
Fibroblast from Lung, Lung
Description:
IMR-91 - NORMAL HUMAN FETAL FIBROBLAST
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
IMR90/IMR91 Cell Cultures
Specially Characterized Fibroblasts |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
15 |
| Passage Frozen |
5 |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this cell culture gave a positive result with a primer for Yq11, DYS227. |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The IMR-91 lung-derived line was initiated on 2/24/76 from explants of minced tissue of both lungs from an approximately 12 week fetus. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The reported number of population doublings achieved to senescence range from 49 to 76. The HLA typing is A2, A11, B18 and B5. G6PD is isoenzyme type B and 6PGD is isoenzyme type A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Ye Q, Zhang Y, Cao Y, Wang X, Guo Y, Chen J, Horn J, Ponomareva LV, Chaiswing L, Shaaban KA, Wei Q, Anderson BD, St Clair DK, Zhu H, Leggas M, Thorson JS, She QB, Frenolicin B Targets Peroxiredoxin 1 and Glutaredoxin 3 to Trigger ROS/4E-BP1-Mediated Antitumor Effects Cell chemical biology: 2018 |
| PubMed ID: 30661989 |
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| Sagesaka T, Boubnov N, Okuyama T, Paulus H, Sarkar N, Deoxyribonucleic acid replication in fetal cells. Am J Obstet Gynecol170(2):468-73 1994 |
| PubMed ID: 8116699 |
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| Mukherjee AB, Wallace KC, Changes in frequency and localization of human X- and Y-chromatin bodies at interphase during in vitro cellular aging. Mech Ageing Dev53(1):61-71 1990 |
| PubMed ID: 2325441 |
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| Green L, Whittle W, Dell'Orco R, Ostrer H, Stein G, Stein J, Human histone gene organization. Identification of a histone gene polymorphism prevalent in a black population. Exp Cell Res164:507-15 1986 |
| PubMed ID: 3011475 |
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| Lusis AJ, Heinzmann C, Sparkes RS, Scott J, Knott TJ, Geller R, Sparkes MC, Mohandas T, Regional mapping of human chromosome 19: organization of genes for plasma lipid transport (APOC1, -C2, and -E and LDLR) and the genes C3, PEPD, and GPI. Proc Natl Acad Sci U S A83:3929-33 1986 |
| PubMed ID: 3459164 |
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| Yen PH, Mohandas T, Shapiro LJ, Stability of DNA methylation of the human hypoxanthine phosphoribosyltransferase gene. Somat Cell Mol Genet12:153-61 1986 |
| PubMed ID: 3008350 |
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| Nichols WW, Cristofalo VJ, Toji LH, Greene AE, Aronson MM, Dwight S, Charpentier R, Hoffman E, Characterization of a new human diploid cell line--IMR-91. In Vitro19:797-804 1983 |
| PubMed ID: 6629383 |
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| Lieberman MA, Raben D, Glaser L, Cell surface-associated growth inhibitory proteins. Exp Cell Res133:413-9 1981 |
| PubMed ID: 7238608 |
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| Bynum JW, Characterization of adenosine-induced cytostasis in melanoma cells. Cancer Res40:2147-52 1980 |
| PubMed ID: 7388782 |
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| Fischer HD, Gonzalez-Noriega A, Sly WS, Beta-glucuronidase binding to human fibroblast membrane receptors. J Biol Chem255:5069-74 1980 |
| PubMed ID: 6768729 |
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| Brown, Two-dimensional analysis of radiolabeled proteins in cultured Huntington's Disease fibroblasts. Adv Neurol23:361 (1979):5069-74 1979 |
| PubMed ID: 6768729 |
| Cumulative PDL at Freeze |
15 |
| Passage Frozen |
5 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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